Biology
06102026–2028 syllabus

BIOLOGY · CHAPTER 3

Movement into and out of cells

How concentration gradients, membranes and energy control particle movement.

Core + Supplement3 connected sectionsSyllabus-aligned guide

LEARNING OBJECTIVES

What you will be able to do

  • explain diffusion and its rate
  • plan diffusion and osmosis investigations
  • describe osmosis using water potential
  • predict effects on plant cells
  • explain active transport and carrier proteins

AT A GLANCE

Syllabus0610Coverage2026–2028Sections3LevelCore + Supplement

INTRODUCTION · THE BIG IDEA

How concentration gradients, membranes and energy control particle movement.

Cells survive only if useful substances enter and wastes leave at suitable rates. Particle motion, concentration gradients and membrane proteins determine how that exchange occurs.

The central comparison is between passive movement down a gradient and active transport against one. Water movement requires the more precise idea of water potential.

01

SECTION 01

Diffusion

Core concept

Particles move randomly. When more particles begin in one region, random movement produces a net movement down the concentration gradient until distribution is more even.

DETAILED EXPLANATION

  • A steeper gradient, higher temperature, larger surface area and shorter distance increase the rate.
  • Energy comes from particles' kinetic energy, not directly from respiration.
  • A diffusion investigation can compare how far a coloured substance travels through agar in a fixed time. Change one factor, such as temperature, while keeping agar size, concentration and time constant.
ORIGINAL STUDY DIAGRAMDown a concentration gradient
1high concentration
2net random movement
3low concentration
02

SECTION 02

Osmosis and water potential

Core concept

Osmosis is the net movement of water from higher water potential in a dilute solution to lower water potential in a concentrated solution through a partially permeable membrane.

DETAILED EXPLANATION

  • Water entering a plant cell makes it turgid; the wall resists expansion and creates turgor pressure.
  • Water loss makes a cell flaccid. Severe loss pulls the membrane from the wall, called plasmolysis.
  • Osmosis supports plants and controls water uptake and loss in organisms.
  • To investigate osmosis, cut equal plant-tissue pieces, record their initial mass or length, place them in different concentrations for the same time, blot them dry and calculate the change. Repeats improve reliability.
RULE 1
percentage change = (final value − initial value) ÷ initial value × 100
ORIGINAL STUDY DIAGRAMPlant cells in solutions
1dilute outside → water enters → turgid
2balanced movement → flaccid
3concentrated outside → water leaves → plasmolysed
03

SECTION 03

Active transport

Core concept

Active transport moves molecules or ions against their concentration gradient through a membrane, using energy released by respiration.

DETAILED EXPLANATION

  • Root hair cells can absorb mineral ions even when the soil contains a lower ion concentration than the cell.
  • Cells performing much active transport need a strong supply of respiratory energy.
ORIGINAL STUDY DIAGRAMAgainst the gradient
1low concentration
2carrier protein + energy
3high concentration

STEP-BY-STEP EXAM EXAMPLE

Original worked example

Interpreting an osmosis investigation

  1. A potato cylinder changes from 4.80 g to 5.28 g.
  2. Find the change: 5.28 − 4.80 = 0.48 g.
  3. Divide by the initial mass: 0.48 ÷ 4.80 = 0.10.
  4. Multiply by 100 and connect the gain to net water entry.

Answer: The mass increased by 10%; water entered the cells by osmosis.

QUICK CHAPTER SUMMARY

The ideas to carry forward

  • Diffusion moves particles down gradients.
  • Osmosis moves water through a partially permeable membrane.
  • Water gain makes plant cells turgid.
  • Active transport uses respiratory energy and carrier proteins.

QUICK REVISION CHECKLIST

Can you do each of these without your notes?

  • explain diffusion and its rate
  • plan diffusion and osmosis investigations
  • describe osmosis using water potential
  • predict effects on plant cells
  • explain active transport and carrier proteins